The following highways are numbered 335:

• New Brunswick Route 335
• Newfoundland and Labrador Route 335
• Nova Scotia Route 335
• Quebec Route 335
• Prince Edward Island Route 335
• Saskatchewan Highway 335

• National Route 335

• National Highway 335 (India)

• Japan National Route 335

• Interstate 335
• County Road 335 (Levy County, Florida)
• Arkansas Highway 335
• Georgia State Route 335
• Indiana State Road 335
• Kentucky Route 335
• Maryland Route 335
• New York:
  • New York State Route 335
  • County Route 335 (Erie County, New York)
• Ohio State Route 335
• Oregon Route 335
• Puerto Rico Highway 335
• Tennessee State Route 335
• Texas:
  • Texas State Highway 335
  • Texas State Highway Loop 335
• Virginia State Route 335
• Wyoming Highway 335