Gustavo Kuerten defeated Andre Agassi in the final, 6–4, 6–4, 6–4 to win the singles tennis title at the 2000 Tennis Masters Cup. With the win, Kuerten became the year-end No. 1, and became the first South American to make the achievement.

Pete Sampras was the defending champion, but was defeated in the semifinals by Kuerten.

	Semifinals							Final
	3	Pete Sampras	77	3	4
	2	Gustavo Kuerten	65	6	6
								2	Gustavo Kuerten	6	6	6
								8	Andre Agassi	4	4	4
	8	Andre Agassi	6	6
	1	Marat Safin	3	3

		Safin	Sampras	Hewitt	Corretja	RR W–L	Set W–L	Game W–L	Standings
1	Marat Safin		3–6, 2–6	6–4, 6–4	6–7(6–8), 7–5, 6–3	2–1	4–3	36–35	2
3	Pete Sampras	6–3, 6–2		5–7, 0–6	7–6(7–2), 7–5	2–1	4–2	31–29	1
6	Lleyton Hewitt	4–6, 4–6	7–5, 6–0		6–3, 6–7(3–7), 3–6	1–2	3–4	36–33	4
7	Álex Corretja	7–6(8–6), 5–7, 3–6	6–7(2–7), 5–7	3–6, 7–6(7–3), 6–3		1–2	3–5	42–48	3

Standings are determined by: 1. number of wins; 2. number of matches; 3. in two-players-ties, head-to-head records; 4. in three-players-ties, percentage of sets won, or of games won; 5. steering-committee decision.

		Kuerten	Norman	Kafelnikov	Agassi	RR W–L	Set W–L	Game W–L	Standings
2	Gustavo Kuerten		7–5, 6–3	6–3, 6–4	6–4, 4–6, 3–6	2–1	5–2	38–31	2
4	Magnus Norman	5–7, 3–6		6–4, 5–7, 1–6	3–6, 2–6	0–3	1–6	25–42	4
5	Yevgeny Kafelnikov	3–6, 4–6	4–6, 7–5, 6–1		1–6, 4–6	1–2	2–5	29–36	3
8	Andre Agassi	4–6, 6–4, 6–3	6–3, 6–2	6–1, 6–4		3–0	6–1	40–23	1

Standings are determined by: 1. number of wins; 2. number of matches; 3. in two-players-ties, head-to-head records; 4. in three-players-ties, percentage of sets won, or of games won; 5. steering-committee decision.

• ATP World Tour Finals appearances

• Draw