Sant'Agnese (or Sant'Agnese in Chianti) is a village in Tuscany, central Italy, administratively a frazione of the comune of Castellina in Chianti, province of Siena. At the time of the 2018 parish census its population was 90.[1]

Sant'Agnese is about 33 km from Siena and 9 km from Castellina in Chianti.

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