John Fleming may refer to:

• John Fleming (14th-century MP) for Rochester
• John Fleming, 1st Earl of Wigtown (1567–1619), Scottish aristocrat and diplomat
• John Fleming, 2nd Lord Fleming (c. 1465–1524), Scottish nobleman
• John Fleming, 5th Lord Fleming (1529–1572), Lord Chamberlain of Scotland, 1565–1572
• John Fleming (American politician) (born 1951), Republican U.S. representative for Louisiana's 4th congressional district
• John Fleming (Canadian politician) (1819–1877), Ontario businessman and political figure
• John Fleming (Devonport MP), 19th-century politician
• John Fleming (Scottish politician) (1847–1925), Liberal MP for Aberdeen South
• John Fleming (Southampton MP) (1743–1802), Tory politician in England
• John Fleming (Gatton and Saltash MP) (1747–1829), British surgeon, naturalist, and politician
• John M. Fleming (1832–1900), American politician and newspaper editor
• John Willis Fleming (1781–1844), MP for Hampshire and South Hampshire

• Jackie Fleming (John Fleming), English rugby league footballer
• John Fleming (footballer, born 1889) (1889–1916), Scottish footballer
• John Fleming (footballer, born 1953), English footballer
• John Fleming (rugby union) (born 1953), New Zealand rugby union player
• John Fleming (sport shooter) (1881–1965), British sport shooter
• John Fleming (1901–1961), Scottish boxer who fought under the name Johnny Brown
• Jock Fleming (1864–1934), Scottish footballer

• John Fleming (art historian) (1919–2001), British art historian
• John Fleming (Australian priest) (born 1943), Australian priest and bioethicist
• John Fleming (bishop) (born 1948), Irish Roman Catholic clergyman
• John Fleming (dean of Ross)
• John Fleming (DJ) (born 1969), English trance producer and DJ
• John Fleming (engineer) (born 1951), Liverpool-born former head of Ford of Europe
• John Fleming (judge) (1697–1766), American judge in Virginia
• John Fleming (naturalist) (1785–1857), Scottish zoologist and geologist
• John Fleming (New York judge) (1842–1918), Queens County district attorney and New York judge
• John Fleming (painter) (1792–1845), Scottish painter
• Sir John Fleming, 1st Baronet, Irish baronet
• John Adam Fleming (1877–1956), American physicist
• John Ambrose Fleming (1849–1945), English electrical engineer and inventor of the Fleming Valve
• John Arnold Fleming (1871–1966), industrial chemist
• John Gibson Fleming (1809–1879), Scottish surgeon and medical administrator
• John Henry Fleming (1816–1894), ringleader of the Myall Creek massacre
• John V. Fleming (born 1936), American literary critic and professor

• John Le Fleming (1865–1942), English cricketer and rugby union player
• John Flammang Schrank (1876–1943), American who attempted to assassinate Theodore Roosevelt
• Jack Fleming (1924–2001), American sports announcer
• John Flemming (1941–2003), English economist and Wadham College warden
• John Flemming (racing driver) (born 1967), Canadian racing driver
• John Fleeming, 18th-century American printer