Bisbenzimide (Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications.[1] Several related chemical compounds are used for similar purposes and are collectively called Hoechst stains.

Application

Bisbenzimide tends to bind to adenine–thymine-rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.

Nuclei of Platynereis dumerilii larvae stained with Hoechst 33342

References

  1. ^ "bisBenzimide H 33342 trihydrochloride". Sigma-Aldrich.
Allikas: https://en.wikipedia.org/wiki/Hoechst_33342
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