Fruit fly may refer to:

• Drosophilidae, a family of small flies, including:
  • Drosophila, the genus of small fruit flies and vinegar flies
  • Drosophila melanogaster or common fruit fly
  • Drosophila suzukii or Asian fruit fly
• Tephritidae, a family of large flies
  • Bactrocera cucurbitae or melon fly
  • Bactrocera oleae or olive fruit fly
  • Bactrocera tryoni or Queensland fruit fly
  • Vidalia (fly), a genus
• Ceratitis capitata, the Mediterranean fruit fly

• Fruit Fly (film), 2009 film directed by H. P. Mendoza
• Fruit (slang) § Fruit fly, LGBT slang with a meaning similar to fag hag