2,3-Dihydroxy-3-methylpentanoic acid is an intermediate in the metabolism of isoleucine.

Metabolism

2,3-Dihydroxy-3-methylpentanoate is synthesized by the action of acetolactate mutase with subsequent reduction from α-aceto-α-hydroxybutyrate through 3-hydroxy-2-keto-3-methylpentanoate:[1]

α-aceto-α-hydroxybutyrate → 3-hydroxy-2-keto-3-methylpentanoate
3-hydroxy-2-keto-3-methylpentanoate + NAD(P)H → 2,3-dihydroxy-3-methylpentanoate + NAD(P)+

It is then processed by the action of dihydroxyacid dehydratase, which results in 2-keto-3-methylvalerate and water:[1]

2,3-dihydroxy-3-methylpentanoate → 2-keto-3-methylvalerate + H2O

Transamination of 2-keto-3-methylvalerate yields isoleucine.

References

  1. ^ a b Voet, Donald; Voet, Judith G. (2011). Biochemistry (4. ed.). Hoboken, NJ: Wiley. pp. 1074–1075. ISBN 978-0-470-91745-9.
Allikas: https://en.wikipedia.org/wiki/2,3-Dihydroxy-3-methylpentanoic_acid
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