TKU may refer to:
Universities
- Tamkang University, a private university in New Taipei, Taiwan
- Tokyo Keizai University, a private university in Kokubunji, Tokyo, Japan
- The King's University (Texas), a private university in Southlake, Texas, United States
- The King's University (Edmonton), a Christian university in Edmonton, Alberta, Canada
Other uses
- Three Kings United, the New Zealand football club
- TV Kumamoto, part of the Fuji News Network
- The Kristet Utseende, a Swedish punk/ metal group
- IATA code for Turku Airport, Finland