The following highways are numbered 416:

• Manitoba Provincial Road 416
• Newfoundland and Labrador Route 416
• Highway 416 (Ontario)

• National Route 416

• Japan National Route 416

• Florida:
  • Florida State Road 416
  • County Road 416 (Pinellas County, Florida)
• Louisiana Highway 416
• Maryland Route 416 (former)
• New York State Route 416
• Ohio State Route 416
• Pennsylvania Route 416
• Puerto Rico Highway 416
• Tennessee State Route 416
• Texas State Highway Loop 416 (former)