Margaret Court defeated Nancy Richey in the final, 6–2, 6–2 to win the women's singles tennis title at the 1969 US Open. It was her third US Open singles title and 16th singles major overall. Court did not lose a set during the tournament. She would go on to win the next five majors, setting the record for most consecutive major titles in the Open Era at six.

Virginia Wade was the defending champion, but lost in the semifinals to Court.

The seeded players are listed below. Margaret Court is the champion; others show the round in which they were eliminated.

1. Ann Haydon-Jones (withdrew from the tournament before it began)
2. Margaret Court (champion)
3. Billie Jean King (quarterfinals)
4. Julie Heldman (quarterfinals)
5. Virginia Wade (semifinals)
6. Nancy Richey (finalist)
7. Rosie Casals (semifinals)
8. Kerry Melville (first round)

• Q = Qualifier
• WC = Wild card
• LL = Lucky loser
• r = Retired

	Quarterfinals							Semifinals							Finals
		Peaches Bartkowicz	2	2
	7	Rosie Casals	6	6
								7	Rosie Casals	5	3
								6	Nancy Richey	7	6
	3	Billie Jean King	4	6
	6	Nancy Richey	6	8
															6	Nancy Richey	2	2
															2	Margaret Court	6	6
	5	Virginia Wade	6	6
	4	Julie Heldman	4	3
								5	Virginia Wade	5	0
								2	Margaret Court	7	6
		Karen Krantzcke	0	7
	2	Margaret Court	6	9

	First round							Second round							Third round							Quarterfinals
		Bartkowicz	6	6
		Lebedeff	1	0					Bartkowicz	6	6
		Bonicelli	6	6					Bonicelli	2	3
		Taylor	3	1												Bartkowicz	6	6
		Hard	5	6	6											Dürr	4	2
		Lundquist	7	3	0				Hard	3	3
		Dürr	6	7					Dürr	6	6
		Richmond	2	5																			Bartkowicz	2	2
	7	Casals	6	6																		7	Casals	6	6
		Arias	2	4				7	Casals	6	4	6
		Bowrey	7	6					Bowrey	3	6	2
		Netter	5	1											7	Casals	6	6
		Overton	7	6												Michel	3	1
		Volavková	3	2					Overton	4	4
		Michel	8	6					Michel	6	6
		Gengler	6	4

	First round							Second round							Third round							Quarterfinals
	3	King	6	8
		Kirk	2	6				3	King	6	6
		Aschner	2	8	6				Aschner	3	4
		Austin	6	6	4										3	King	6	6
		Teeguarden	6	8												Carter	1	3
		Hatanaka	3	6					Teeguarden	6	6	3
		Carter	6	6					Carter	8	2	6
		Tym	4	0																		3	King	4	6
	6	Richey	6	6																		6	Richey	6	8
		Reese	2	1				6	Richey	6	6
		Williams	9	6					Williams	3	1
		Walsh	7	0											6	Richey	3	6	6
		Shaw	6	6												Shaw	6	4	2
		Curtis	3	4					Shaw	6	6
		Chanfreau	6	8					Chanfreau	3	1
		Pigeon	3	6

	First round							Second round							Third round							Quarterfinals
		Tomlinson	6	4	7
		Hansen	3	6	5				Tomlinson	2	4
		Hunt	6	6					Hunt	6	6
		Hogan	2	3												Hunt	7	0
		Capozzi	6	3	6										5	Wade	9	6
		Amos	3	6	1				Capozzi	1	1
	5	Wade	6	6				5	Wade	6	6
		Kemmer	1	4																		5	Wade	6	6
		Ziegenfuss	6	6																		4	Heldman	4	3
		O'Shaughnessy	0	1					Ziegenfuss	6	8
		Tuero	7	6					Tuero	1	6
		Aucamp	5	3												Ziegenfuss	2	4
		Urban	6	6											4	Heldman	6	6
		Susman	3	4					Urban	1	1
	4	Heldman	6	3	8			4	Heldman	6	6
		Truman	4	6	6

	First round							Second round							Third round							Quarterfinals
		Young	6	6
		Christenson	3	4					Young	3	3
		Krantzcke	6	6					Krantzcke	6	6
		Ornstein	3	3												Krantzcke	7	6
		Martinez	6	6												Janes	5	2
		Grant	3	3					Martinez	1	2
		Janes	8	6					Janes	6	6
	8	Melville	6	4																			Krantzcke	0	7
		Graebner	6	6																		2	Court	6	9
		Cavadini	2	4					Graebner	3	4
		Burrer	6	6					Burrer	6	6
		Harris	4	2												Burrer	0	2
		Grubb	6	6											2	Court	6	6
		Emmanuel	3	2					Grubb	1	0
	2	Court	6	6				2	Court	6	6
		Petersen	1	0

• 1969 US Open – Women's draws and results at the International Tennis Federation