Eisner (/ˈaɪznər/ EYEZ-nər, German: [ˈaɪsnɐ]) or Eissner is a surname. Notable people with the name include:

• Alexey Eisner (1905–1984), a Russian poet, translator and writer
• Ali Eisner (born 1973), a Canadian composer, director and photographer for children's television
• Bertold Eisner (1875–1956), Croatian law professor, pioneer of Croatian jurisprudence and writer
• Betty Grover Eisner (1915–2004), an American psychologist, pioneering researcher on LSD and psychedelic drugs
• Brian Eisner (born c. 1942), American tennis player and coach
• Breck Eisner, son of Michael Eisner and film director
• Bruce Eisner (1948–2013), pen name of Bruce Jay Ehrlich, American psychologist and author of the book Ecstasy: The MDMA Story
• Clara Eissner, see Clara Zetkin
• Curt Eisner (1890–1981), a German entomologist, specialised in snow butterflies
• David A. Eisner, British physiologist
• David Eisner (born 1958), Canadian actor
• Edward Eisner (1929–1987), Hungarian-born physicist
• Elliot W. Eisner, Professor of Art and Education at Stanford University
• Eric Eisner, son of Michael and film producer
• Eric Eisner (lawyer), former head of The Geffen Film Company
• Gilbert Eisner, American fencer
• Herbert Sigmund Eisner (1921–2011), British-German physicist and playwright
• Hubert Eisner (1897–date of death unknown), Austrian Nazi and became Kreisleiter of Voitsberg
• Ib Eisner (1925–2003), Danish artist
• Jacob Eisner (born 1947), Israeli basketball player
• Kurt Eisner (1867–1919), German-Jewish journalist, leader of the 1918 revolution in Bavaria
• Lotte H. Eisner (1896?–1983), German writer
• Manuel Eisner, British academic and researcher on the history of interpersonal violence
• Mark Eisner, American prominent lawyer, tax expert, and politician
• Maria Eisner (1909–1991), Italian photographer
• Michael Eisner, former head of The Walt Disney Company
• Pavel Eisner (or Paul Eisner; 1889–1958), Czech-German linguist and translator
• Philip Eisner, American screenwriter, author of the screenplay for the 1997 film Event Horizon
• Shiri Eisner, Israeli writer and activist
• Sigmund Eisner, Austrian entrepreneur
• Sigmund Eisner (academic) (1920–2012), American scholar of medieval literature
• Simon Eisner, Swedish philanthropist
• Sol Eisner, American soccer player
• Tanja Eisner (born 1980), Ukrainian and German mathematician
• Thomas Eisner, chemical ecologist and author of several books, including For the Love of Insects
• Will Eisner (1917–2005), comics artist and writer, best known as the creator of The Spirit