Autopsy is a Greek word meaning 'seeing for yourself', and is usually employed in a medical sense, referring to the examination of a corpse in order to determine cause of death.

Autopsy may also refer to:

• Autopsy (1975 film), Italian film
• Autopsy (2008 film), American horror film
• The Autopsy of Jane Doe, a 2016 film

• Autopsy (band), death metal band
• Autopsy (45 Grave album), 1987
• Autopsy (Rorschach album), 1995
• The Autopsy, a 1994 album by American rapper C-Bo
• "Autopsy", a song by Fairport Convention from their 1969 album Unhalfbricking

• "Autopsy" (House), 2005 episode of the American television series House
• Autopsy (TV series), American documentary television series
• “Autopsy”, an episode of The Good Doctor

• Autopsy (software), open source computer forensics software tool

• Post-mortem (disambiguation)