The 2021 Challenger Concepción was a professional tennis tournament played on clay courts. It was the first edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Concepción, Chile between 15 and 21 February 2021.

Country	Player	Rank1	Seed
ARG	Federico Coria	92	1
SVK	Andrej Martin	103	2
POR	Pedro Sousa	107	3
BOL	Hugo Dellien	112	4
COL	Daniel Elahi Galán	115	5
BRA	Thiago Seyboth Wild	117	6
ARG	Facundo Bagnis	127	7
GER	Daniel Altmaier	134	8

• ¹ Rankings as of 8 February 2021.^[1]

The following players received wildcards into the singles main draw:

• Nicolás Álvarez
• Nicolás Jarry
• Gonzalo Lama

The following player received entry into the singles main draw as an alternate:

• Camilo Ugo Carabelli

The following players received entry from the qualifying draw:

• Hernán Casanova
• Carlos Gómez-Herrera
• Vitaliy Sachko
• Thiago Agustín Tirante

• Sebastián Báez def. Francisco Cerúndolo 6–3, 6–7^(5–7), 7–6^(7–5).

• Orlando Luz / Rafael Matos def. Sergio Galdós / Diego Hidalgo 7–5, 6–4.