The 2016 Kentucky Bank Tennis Championships was a professional tennis tournament played on outdoor hard courts. It was the 21st edition, for men, and 19th edition, for women, of the tournament and part of the 2016 ATP Challenger Tour and the 2016 ITF Women's Circuit, offering totals of $50,000, for both genders, in prize money. It took place in Lexington, Kentucky, United States, on 25–31 July 2016.

Country	Player	Rank1	Seed
FRA	Adrian Mannarino	55	1
TUN	Malek Jaziri	63	2
GBR	Daniel Evans	82	3
AUS	Jordan Thompson	90	4
USA	Bjorn Fratangelo	108	5
FRA	Quentin Halys	140	6
USA	Frances Tiafoe	156	7
USA	Stefan Kozlov	163	8

• ¹ Rankings as of 18 July 2016.

The following players received a wildcard into the singles main draw:

• Brian Baker
• Eric Quigley
• Ryan Shane
• William Bushamuka

The following player entered as an alternate:

• Juan Ignacio Londero

The following players received entry from the qualifying draw:

• Lloyd Glasspool
• Alex Kuznetsov
• Mackenzie McDonald
• Jesse Witten

Country	Player	Rank1	Seed
BEL	Alison Van Uytvanck	116	1
CZE	Kristýna Plíšková	119	2
ROU	Ana Bogdan	135	3
USA	Taylor Townsend	148	4
JPN	Hiroko Kuwata	154	5
CHN	Zhu Lin	151	6
USA	Grace Min	158	7
JPN	Miyu Kato	182	8
BEL	An-Sophie Mestach	189	9

• ¹ Rankings as of 18 July 2016.

The following players received a wildcard into the singles main draw:

• Francesca Di Lorenzo
• Raveena Kingsley
• Jamie Loeb
• Melanie Oudin

The following players received entry from the qualifying draw:

• Alison Bai
• Cristiana Ferrando
• Greet Minnen
• Olivia Rogowska

The following players received entry as lucky losers:

• Katy Dunne
• Laura Robson
• Emily Webley-Smith

• Ernesto Escobedo def. Frances Tiafoe, 6–2, 6–7^(6–8), 7–6^(7–3)

• Michaëlla Krajicek def. Arina Rodionova, 6–0, 2–6, 6–2

• Luke Saville / Jordan Thompson def. Nicolaas Scholtz / Tucker Vorster, 6–2, 7–5

• Hiroko Kuwata / Zhu Lin def. Sophie Chang / Alexandra Mueller, 6–0, 7–5

• 2016 Kentucky Bank Tennis Championships at ITFtennis.com
• Official website