GENtle is a free software under GPL license.^[1]

GENtle is an equivalent to the proprietary Vector NTI, a tool for molecular biologists to analyze and edit DNA sequence files. Invitrogens' removal of the free-of-cost academic licence for Vector NTI v11 has had a severe impact on many molecular biology labs that have come to rely on that tool, which led to vendor lock-in effects, which angered many molecular biologists.^{[citation needed]} The GENtle code is developed and maintained^[2] by Magnus Manske. By design, GENtle is coded to be cross-platform utilizing wxGTK.