A hornets' nest is a place of refuge for hornets.

Hornets Nest or hornet's nest may also refer to:

• Charlotte, North Carolina, nicknamed The Hornet's Nest and home to the Charlotte Hornets

• The Hornet's Nest (1919), American drama film directed by James Young
• Hornet's Nest (1923 film), British drama film directed by Walter West
• The Hornet's Nest (1955 film), British comedy film
• Hornets' Nest (1970), an Italian-American war film directed by Phil Karlson
• Hornet's Nest (novel), a 1997 novel
• The Hornet's Nest (novel), a 2003 novel by former president Jimmy Carter
• Hornets' Nest (audio drama), a 2009 Doctor Who audio play
• The Hornet's Nest (2014 film), an American documentary film about the Afghanistan War

• Hornet's Nest (Civil War), a salient held by the Union army in the 1862 Battle of Shiloh
• Hornets Nest (Sacramento State), a gymnasium at California State University, Sacramento
• Hornet's Nest, a Palestinian militant/insurgent group operating in the West Bank, affiliated with the Al-Aqsa Martyrs' Brigades

• The Girl Who Kicked the Hornets' Nest (2007), the third novel in Stieg Larsson's Millennium series