Langhorne may refer to:

• Langhorne Borough, Pennsylvania
  • Langhorne Speedway
  • Langhorne (SEPTA station)
• Langhorne Manor, Pennsylvania, borough in Bucks County, Pennsylvania
• Langhorne Creek, South Australia

Given name

• Langhorne Bond (1937–2022), U.S. federal administrator
• Langhorne Slim (born 1980), American country singer
• Langhorne Wister (1834–1891), American military officer

Surname

• Bruce Langhorne (1938–2017), American folk musician
• Cary DeVall Langhorne (1873–1948), American Medal of Honor recipient
• Crystal Langhorne (born 1986), American basketball player
• Francis Harold Langhorne (1892–1918), Lieutenant in the 2nd Canadian Mounted Rifles killed in action at Cambrai
• Harold Stephen Langhorne (1866–1932), Brigadier-General in the British Army in the First World War
• James Archibald Dunboyne Langhorne (1878–1950), Brigadier in the British Army
• Jeremiah Langhorne (died 1742), prominent landowner and jurist in colonial Pennsylvania
• John Langhorne (poet) (1735–1779), English poet and clergyman
• John Langhorne (1805–1881), mathematics master of Giggleswick School
• John Langhorne (1862–1925), master of Loretto School and headmaster of the Dean Orphanage Charity School later known as the John Watson's Institution in Edinburgh
• Reverend John Langhorne (1836–1911), master of Tonbridge School and headmaster of The King's School, Rochester
• Michael Langhorne Astor (1916–1980), British politician
• Nancy Witcher Langhorne, bka Nancy Astor (1879–1964), American-born first woman British MP
• Reggie Langhorne (born 1963), American football player
• Samuel Langhorne Clemens (1835–1910), author, pen name Mark Twain
• Reverend Thomas Langhorne (born 1797), founder of Loretto School
• Sir William Langhorne, 1st Baronet (c. 1631–1715), merchant, landowner and Governor of Madras