Ligature may refer to:

• Ligature (writing), a combination of two or more letters into a single symbol (typography and calligraphy)
• Ligature (grammar), a morpheme that links two words

• Ligature (medicine), a piece of suture used to shut off a blood vessel or other anatomical structure
  • Ligature (orthodontic), used in dentistry

• Ligature (music), an element of musical notation used especially in the medieval and Renaissance periods
• Ligature (instrument), a device used to attach a reed to the mouthpiece of a woodwind instrument

• Ligature strangulation