The enzyme itaconyl-CoA hydratase (EC 4.2.1.56) catalyzes the chemical reaction

citramalyl-CoA itaconyl-CoA + H₂O

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is citramalyl-CoA hydro-lyase (itaconyl-CoA-forming). Other names in common use include itaconyl coenzyme A hydratase, and citramalyl-CoA hydro-lyase. This enzyme participates in c5-branched dibasic acid metabolism.

• Cooper RA, Kornberg HL (1964). "The utilization of itaconate by Pseudomonas sp". Biochem. J. 91 (1): 82–91. PMC 1202819. PMID 4284209.