The 2022 Cali Open was a professional tennis tournament played on clay courts. It was the first edition of the tournament which was part of the 2022 ATP Challenger Tour. It took place in Cali, Colombia between 27 June and 2 July 2022.

Country	Player	Rank1	Seed
CHI	Tomás Barrios Vera	131	1
ARG	Facundo Mena	162	2
ARG	Juan Pablo Ficovich	168	3
BRA	Felipe Meligeni Alves	213	4
ARG	Francisco Comesaña	239	5
AUT	Gerald Melzer	245	6
CHI	Gonzalo Lama	250	7
SRB	Miljan Zekić	271	8

• ¹ Rankings as of 20 June 2022.^[1]

The following players received wildcards into the singles main draw:

• Tomás Barrios Vera
• Mateo Gómez
• Andrés Urrea

The following players received entry into the singles main draw as alternates:

• Michail Pervolarakis
• Evan Zhu

The following players received entry from the qualifying draw:

• Mateus Alves
• Román Andrés Burruchaga
• Nick Chappell
• Chung Yun-seong
• Juan Bautista Otegui
• Matías Zukas

• Facundo Mena def. Miljan Zekić 6–2, 7–6^(7–3).

• Malek Jaziri / Adrián Menéndez Maceiras def. Keegan Smith / Evan Zhu 7–5, 6–4.