Dinara Safina defeated Svetlana Kuznetsova in the final, 6–3, 6–2 to win the women's singles tennis title at the 2009 Italian Open.

Jelena Janković was the two-time defending champion, but lost in the quarterfinals to Kuznetsova.

The top eight seeds receive a bye into the second round.

	Quarterfinals							Semifinals							Final
	1	Dinara Safina	4	6	6
		María José Martínez Sánchez	6	3	0
								1	Dinara Safina	63	6	6
								4	Venus Williams	7	3	4
	4	Venus Williams	6	6
	10	Agnieszka Radwańska	1	2
															1	Dinara Safina	6	6
															7	Svetlana Kuznetsova	3	2
	7	Svetlana Kuznetsova	6	7
	3	Jelena Janković	1	63
								7	Svetlana Kuznetsova	6	6
								6	Victoria Azarenka	2	4
	6	Victoria Azarenka	7	6
	16	Kaia Kanepi	65	3

	First round							Second round							Third round							Quarterfinals
								1	D Safina	7	6
		V Razzano	6	68	6				V Razzano	61	1
		M Rybáriková	0	7	4										1	D Safina	5	6	7
		E Vesnina	1	6	6										14	J Zheng	7	1	63
	Q	J Craybas	6	3	2				E Vesnina	2	7	2
		N Dechy	1	64				14	J Zheng	6	63	6
	14	J Zheng	6	7																		1	D Safina	4	6	6
	11	M Bartoli	6	7																			MJ Martínez Sánchez	6	3	0
		S Peng	0	63				11	M Bartoli	1	5
		A Mauresmo	6	64	2				MJ Martínez Sánchez	6	7
		MJ Martínez Sánchez	4	7	6											MJ Martínez Sánchez	6	65	6
		A Keothavong	65	4											8	N Petrova	4	7	4
		C Suárez Navarro	7	6					C Suárez Navarro	2	6	3
								8	N Petrova	6	3	6

	First round							Second round							Third round							Quarterfinals
								4	V Williams	6	4	6
		L Šafářová	7	6					L Šafářová	2	6	2
	Q	M Koryttseva	65	2											4	V Williams	6	66	6
		A Wozniak	6	3	6											A Chakvetadze	0	7	4
		S Errani	1	6	4				A Wozniak	7	62	3
		A Chakvetadze	6	6					A Chakvetadze	5	7	6
	15	A Medina Garrigues	2	3																		4	V Williams	6	6
	10	A Radwańska	6	6																		10	A Radwańska	1	2
		A Bondarenko	0	1				10	A Radwańska	4	6	7
		A Kleybanova	3	3				Q	A Rezaï	6	2	67
	Q	A Rezaï	6	6											10	A Radwańska	6	3	6
		F Schiavone	6	6											5	A Ivanovic	1	6	4
	Q	A Brianti	3	4					F Schiavone	3	4
								5	A Ivanovic	6	6

	First round							Second round							Third round							Quarterfinals
								7	S Kuznetsova	6	6
		V Dushevina	4	1					D Hantuchová	3	3
		D Hantuchová	6	6											7	S Kuznetsova	6	3	6
	Q	Y Shvedova	6	6											12	F Pennetta	3	6	0
		S Stosur	4	2				Q	Y Shvedova	3	7	1
		T Pironkova	7	4	1			12	F Pennetta	6	65	6
	12	F Pennetta	62	6	6																	7	S Kuznetsova	6	7
	13	A Cornet	6	7																		3	J Janković	1	63
		A Sugiyama	3	5				13	A Cornet	4	1
	WC	R Vinci	3	64					K Bondarenko	6	6
		K Bondarenko	6	7												K Bondarenko	1	0	r
	WC	K Knapp	2	3											3	J Janković	6	1
		G Dulko	6	6					G Dulko	3	66
								3	J Janković	6	7

	First round							Second round							Third round							Quarterfinals
								6	V Azarenka	6
		M Niculescu	1	3				Q	A Morita	0	r
	Q	A Morita	6	6											6	V Azarenka	6	6
	WC	T Garbin	6	3	1										9	C Wozniacki	2	2
		B Mattek-Sands	3	6	6				B Mattek-Sands	1	5
		Á Szávay	3	2				9	C Wozniacki	6	7
	9	C Wozniacki	6	6																		6	V Azarenka	7	6
	16	K Kanepi	6	6																		16	K Kanepi	65	3
	Q	V King	4	3				16	K Kanepi	7	6
		S Bammer	7	4	6				S Bammer	5	3
		N Li	63	6	3										16	K Kanepi	6	6
		P Schnyder	7	6												P Schnyder	3	0
	Q	M Duque Mariño	61	3					P Schnyder	6	2	6
								2	S Williams	2	6	1

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