Juan Carlos Ferrero defeated Gustavo Kuerten in the final, 3–6, 6–1, 2–6, 6–4, 6–2 to win the men's singles tennis title at the 2001 Italian Open.

Magnus Norman was the defending champion, but lost in the first round to Vincenzo Santopadre.

	Semifinals							Final
	1	Gustavo Kuerten	6	6
		Andreas Vinciguerra	2	4
								1	Gustavo Kuerten	6	1	6	4	2
								8	Juan Carlos Ferrero	3	6	2	6	6
		Nicolás Lapentti	3	3
	8	Juan Carlos Ferrero	6	6

	First round							Second round							Third round							Quarterfinals
	1	G Kuerten	64	7	6
	Q	I Ljubičić	77	5	4			1	G Kuerten	6	6
		F Vicente	4	5					M Chang	1	1
		M Chang	6	7											1	G Kuerten	6	6
	Q	A Voinea	3	4											15	F Squillari	1	2
		F Santoro	6	6					F Santoro	4	0
		M Mirnyi	1	65				15	F Squillari	6	6
	15	F Squillari	6	77																		1	G Kuerten	6	6
	10	À Corretja	5	7	6																	10	À Corretja	2	3
		C Moyá	7	5	1			10	À Corretja	64	6	6
		N Escudé	65	4				Q	A Portas	77	2	4
	Q	A Portas	77	6											10	À Corretja	77	6
		T Haas	7	6											7	L Hewitt	62	4
		A Medvedev	5	3					T Haas	5	3
		B Ulihrach	1	6	5			7	L Hewitt	7	6
	7	L Hewitt	6	2	7

	First round							Second round							Third round							Quarterfinals
	4	P Sampras	5	6	4
		H Levy	7	2	6				H Levy	6	78
		F Clavet	6	6					F Clavet	4	66
		R Schüttler	1	2												H Levy	6	6
		N Kiefer	6	6												N Kiefer	2	4
		C Pioline	3	0					N Kiefer	77	4	6
		G Pozzi	2	1				13	T Enqvist	64	6	3
	13	T Enqvist	6	6																			H Levy	67	2
	12	S Grosjean	6	6																			A Vinciguerra	79	6
		A Ilie	4	2				12	S Grosjean	7	3	7
	Q	S Bruguera	77	77				Q	S Bruguera	5	6	5
	WC	F Volandri	62	63											12	S Grosjean	2	3
		A Vinciguerra	6	6												A Vinciguerra	6	6
	Q	F Meligeni	2	0					A Vinciguerra	3	7	6
	WC	V Santopadre	6	6				WC	V Santopadre	6	5	3
	5	M Norman	4	3

	First round							Second round							Third round							Quarterfinals
	6	Y Kafelnikov	7	3
		A Pavel	5	1	r			6	Y Kafelnikov	63	6	5
	Q	J Díaz	6	6				Q	J Díaz	77	1	7
		M Puerta	1	2											Q	J Díaz	6	6
		V Voltchkov	2	3											WC	F Luzzi	3	1
		H Arazi	6	6					H Arazi	3	64
	WC	F Luzzi	6	1	6			WC	F Luzzi	6	77
	11	A Clément	2	6	3																	Q	J Díaz	6	1	2
	14	J-M Gambill	5	4																			N Lapentti	3	6	6
		G Coria	7	6					G Coria	6	3	5
		N Lapentti	6	6					N Lapentti	3	6	7
		G Gaudio	3	2												N Lapentti	6	2	77
	Q	F Mantilla	6	77											Q	F Mantilla	3	6	64
	WC	M Navarra	3	65				Q	F Mantilla	3	6	77
		Á Calatrava	6	6					Á Calatrava	6	3	63
	3	A Agassi	3	3

	First round							Second round							Third round							Quarterfinals
	8	J C Ferrero	6	7
		S Schalken	3	5				8	J C Ferrero	6	6
		D Sanguinetti	6	3	4				M Ríos	1	3
		M Ríos	4	6	6										8	J C Ferrero	6	6
		T Martin	3	3											Q	J Boutter	3	2
	Q	J Boutter	6	6				Q	J Boutter	2	6	6
		A Costa	2	6	1			9	T Henman	6	3	4
	9	T Henman	6	3	6																	8	J C Ferrero	6	6
	16	D Hrbatý	2	4																			W Ferreira	0	2
		W Ferreira	6	6					W Ferreira	6	6
		G Rusedski	6	6					G Rusedski	4	4
		D Prinosil	3	2												W Ferreira	77	6
		R Federer	77	4	77											R Federer	64	2
		T Johansson	63	6	65				R Federer	4	6	77
		J Björkman	2	6	5			2	M Safin	6	4	65
	2	M Safin	6	3	7

• 2001 Rome Masters draw
• 2001 Rome Masters Qualifying draw