The 2000–01 Copa Federación de España was the eighth staging of the Copa Federación de España, a knockout competition for Spanish football clubs in Segunda División B and Tercera División.
The Regional stages began in 2000, while the national tournament took place from November 2000 to April 2001.
Regional tournaments
| First round | Quarter-finals | Semi-finals | Final | |||||||||||||||||||
| Siero | 1 | 1 | 2 | |||||||||||||||||||
| Marino Luanco | 5 | 1 | 6 | |||||||||||||||||||
| Marino Luanco | 1 | 1 | 2 | |||||||||||||||||||
| Avilés | 0 | 1 | 1 | |||||||||||||||||||
| Sporting Gijón B | 0 | 1 | 1 | |||||||||||||||||||
| Marino Luanco | 1 | 2 | 3 | |||||||||||||||||||
| Sporting Gijón B | 5 | 0 | 5 | |||||||||||||||||||
| Oviedo B | 0 | 2 | 2 | |||||||||||||||||||
| San Martín | 0 | 0 | 0 | |||||||||||||||||||
| Sporting Gijón B | 3 | 2 | 5 | |||||||||||||||||||
| Marino Luanco | 5 | |||||||||||||||||||||
| Llanes | 0 | |||||||||||||||||||||
| Astur | 1 | 1 | 2 | |||||||||||||||||||
| Universidad Oviedo (a) | 2 | 0 | 2 | |||||||||||||||||||
| Navia | 1 | 3 | 4 | |||||||||||||||||||
| Universidad Oviedo | 4 | 2 | 6 | |||||||||||||||||||
| Universidad Oviedo | 2 | 2 | 4 | |||||||||||||||||||
| Llanes (a) | 1 | 3 | 4 | |||||||||||||||||||
| Llanes | 1 | 1 | 2 | |||||||||||||||||||
| Caudal | 0 | 1 | 1 | |||||||||||||||||||
| Caudal | 1 | 1 | 2 | |||||||||||||||||||
| Langreo | 0 | 1 | 1 | |||||||||||||||||||
National tournament
Preliminary round
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg |
|---|---|---|---|---|
Pontevedra 
|
3–4 |  Lealtad
|
0–2 | 3–2 |
| Marino Luanco
|
6–0 | Xove Lago
|
4–0 | 2–0 |
Lemona 
|
0–1 |  Tropezón
|
0–1 | 0–0 |
| Amurrio
|
1–2 | Noja
|
1–1 | 0–1 |
Peña Sport 
|
1–2 |  Calahorra
|
0–0 | 1–2 |
Fraga 
|
2–7 | Zaragoza B
|
0–6 | 2–1 |
Sabadell 
|
4–0 | Balaguer
|
1–0 | 3–0 |
Atlético Baleares 
|
1–2 | Terrassa
|
1–1 | 0–1 |
San Isidro 
|
2–4 |  La Bañeza
|
2–1 | 0–3 |
| Arandina
|
0–11 | Coslada
|
0–3 | 0–8 |
Burjassot 
|
3–4 |  Cartagonova
|
2–2 | 1–2 |
San Fernando 
|
1–2 |  Jerez
|
1–1 | 0–1 |
Tomelloso 
|
0–1 | Don Benito
|
0–0 | 0–1 |
| Olímpico Totana
|
w.o. | Hércules
|
- Castellón and Puertollano received a bye.
Round of 16
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg |
|---|---|---|---|---|
| Tropezón
|
4–3 | Lealtad
|
1–1 | 3–2 |
| Noja
|
1–3 | Marino Luanco
|
0–1 | 1–2 |
| Sabadell
|
1–2 | Zaragoza B
|
0–1 | 1–1 |
| Terrassa
|
3–3 (a) | Calahorra
|
1–1 | 2–2 |
| Olímpico Totana
|
3–8 | Puertollano
|
2–0 | 1–8 |
| Cartagonova
|
2–3 | Castellón
|
1–2 | 1–1 |
Coslada 
|
0–1 | Don Benito
|
0–0 | 0–1 |
| Jerez
|
3–4 | La Bañeza
|
2–2 | 1–2 |
Quarter-finals
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg |
|---|---|---|---|---|
| Marino Luanco
|
3–2 | La Bañeza
|
2–0 | 1–2 |
| Tropezón
|
2–1 | Zaragoza B
|
2–0 | 0–1 |
| Castellón
|
4–2 | Terrassa
|
2–0 | 2–2 |
| Don Benito
|
1–2 | Puertollano
|
1–1 | 0–1 |
Semifinals
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg |
|---|---|---|---|---|
| Marino Luanco
|
4–4 (a) | Puertollano
|
1–1 | 3–3 |
| Castellón
|
0–2 | Tropezón
|
0–1 | 0–1 |
Final
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg |
|---|---|---|---|---|
| Tropezón
|
0–4 | Marino Luanco
|
0–1 | 0–3 |
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