The following highways are numbered 325:

• Nova Scotia Route 325
• Quebec Route 325

• China National Highway 325

• National Route 325

• National Highway 325 (India)

• Japan National Route 325

• Arkansas Highway 325
• Colorado State Highway 325
• Florida State Road 325 (former)
• Georgia State Route 325
• Iowa Highway 325 (former)
• Louisiana Highway 325 (former)
• Kentucky Route 325
• Montana Secondary Highway 325
• New Mexico State Road 325
• New York:
  • New York State Route 325
  • County Route 325 (Erie County, New York)
• Ohio State Route 325
• Oklahoma State Highway 325
• Pennsylvania Route 325
• Puerto Rico Highway 325
• Tennessee State Route 325
• Texas:
  • Texas State Highway 325 (former)
  • Texas State Highway Spur 325
  • Farm to Market Road 325
• Virginia State Route 325