Venus Williams defeated Jennifer Capriati in the final, 4–6, 6–1, 7–6^(7–4) to win the women's singles tennis title at the 2001 Miami Open. Williams saved eight championship points in the third set.^[1][2][3]

Martina Hingis was the defending champion, but lost in the semifinals to Venus Williams.

A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated. All thirty-two seeds received a bye to the second round.

	Quarterfinals							Semifinals							Final
	1	Martina Hingis	7	6
	10	Anke Huber	5	0
								1	Martina Hingis	3	66
								3	Venus Williams	6	78
	3	Venus Williams	6	6
	21	Jelena Dokić	2	3
															3	Venus Williams	4	6	77
															4	Jennifer Capriati	6	1	64
	5	Serena Williams	1	65
	4	Jennifer Capriati	6	77
								4	Jennifer Capriati	6	6
								7	Elena Dementieva	2	0
	7	Elena Dementieva	6	1
	2	Lindsay Davenport	3	0r

	First round							Second round							Third round							Fourth round
								1	M Hingis	6	6
		R Grande	6	7					R Grande	2	2
		S Plischke	4	5											1	M Hingis	6	6
		A Smashnova	5	2											29	T Panova	1	0
	Q	J Craybas	7	6				Q	J Craybas	3	5
								29	T Panova	6	7
																						1	M Hingis	6	6
																						20	M Serna	3	4
								20	M Serna	6	6
	Q	J-Q Yi	4	2					A Glass	3	2
		A Glass	6	6											20	M Serna	6	6
	Q	A Molik	2	4											15	A Frazier	2	1
		Á Montolio	6	6					Á Montolio	6	2	5
								15	A Frazier	2	6	7

	First round							Second round							Third round							Fourth round
								10	A Huber	77	77
		D Chládková	6	6					D Chládková	65	62
		T Poutchek	2	0											10	A Huber	6	6
		M Weingärtner	6	77											24	A Kremer	4	3
		N Petrova	1	65					M Weingärtner	4	7	3
								24	A Kremer	6	5	6
																						10	A Huber	2	6	6
																						8	N Tauziat	6	4	4
								25	H Nagyová	3	6	6
		J Kruger	0	3				Q	S Foretz	6	4	4
	Q	S Foretz	6	6											25	H Nagyová	2	0r
	Q	T Perebiynis	6	2	6										8	N Tauziat	6	5
		A Myskina	3	6	1			Q	T Perebiynis	2	63
								8	N Tauziat	6	77

	First round							Second round							Third round							Fourth round
								3	V Williams	6	6
		M Oremans	4	6	6				M Oremans	2	0
	WC	A Harkleroad	6	4	3										3	V Williams	6	6
		M Tu	5	4											Q	S Cacic	2	2
	Q	S Cacic	7	6				Q	S Cacic	6	6
								27	P Schnyder	2	4
																						3	V Williams	7	77
																							T Garbin	5	62
								22	E Likhovtseva	2	3
		B Rippner	0	0					T Garbin	6	6
		T Garbin	6	6												T Garbin	6	6
	WC	E Bovina	6	77											14	J Henin	3	1
		R Dragomir-Ilie	1	65				WC	E Bovina	2	2
								14	J Henin	6	6

	First round							Second round							Third round							Fourth round
								12	M Maleeva	4	3
		F Schiavone	1	2					M Marrero	6	6
		M Marrero	6	6												M Marrero	2	1
		S Jeyaseelan	2	1											21	J Dokić	6	6
		M Vavrinec	6	6					M Vavrinec	6	3	4
								21	J Dokić	3	6	6
																						21	J Dokić	6	7
																						6	A Coetzer	3	5
								30	K Brandi	4	4
	Q	M J Martínez	3	4					I Majoli	6	6
		I Majoli	6	6												I Majoli	3	3
		R Kuti-Kis	1	5											6	A Coetzer	6	6
	WC	M Irvin	6	7				WC	M Irvin	1	77	3
								6	A Coetzer	6	65	6

	First round							Second round							Third round							Fourth round
								5	S Williams	6	6
		P Nola	7	6					P Nola	1	4
		S Pitkowski	5	0											5	S Williams	3	6	6
		I Tulyaganova	6	1												I Tulyaganova	6	3	2
		F Li	1	0r					I Tulyaganova	714	6
								26	A-G Sidot	612	3
																						5	S Williams	6	6
																						11	K Clijsters	0	2
								23	G León García	4	6	6
		K Habšudová	6	77					K Habšudová	6	2	1
	WC	M Lučić	3	63											23	G León García	3	1
		V Ruano Pascual	6	6											11	K Clijsters	6	6
		S Asagoe	3	0					V Ruano Pascual	2	6	5
								11	K Clijsters	6	2	7

	First round							Second round							Third round							Fourth round
								16	B Schett	4	5
		K Hrdličková	6	2	6				K Hrdličková	6	7
		J Kandarr	2	6	3											K Hrdličková	2	6	4
	Q	V Razzano	3	2											19	T Tanasugarn	6	4	6
	Q	A Kapros	6	6				Q	A Kapros	64	1
								19	T Tanasugarn	77	6
																						19	T Tanasugarn	4	0
																						4	J Capriati	6	6
								31	C Black	64	66
	Q	M Díaz Oliva	7	3	6			Q	M Díaz Oliva	77	78
		Å Carlsson	5	6	2										Q	M Díaz Oliva	1	1
		J Hopkins	6	6											4	J Capriati	6	6
	WC	G Dulko	3	3					J Hopkins	4	0
								4	J Capriati	6	6

	First round							Second round							Third round							Fourth round
								7	E Dementieva	6	2
		M de Swardt	64	4				WC	A Stevenson	2	0r
	WC	A Stevenson	77	6											7	E Dementieva	6	6
	WC	D Hantuchová	6	67	4										32	S Talaja	4	2
		J Nejedly	1	79	6				J Nejedly	3	4
								32	S Talaja	6	6
																						7	E Dementieva	6	6
																						9	A Sánchez Vicario	1	2
								17	M Shaughnessy	2	6	2
	Q	M Palaveršić	3	2					N Pratt	6	2	6
		N Pratt	6	6												N Pratt	0	64
		T Pisnik	77	3	0										9	A Sánchez Vicario	6	77
		B Rittner	63	6	6				B Rittner	2	6	0
								9	A Sánchez Vicario	6	2	6

	First round							Second round							Third round							Fourth round
								13	S Testud	6	6
	WC	M Washington	7	6				WC	M Washington	1	3
		A Gerši	5	1											13	S Testud	6	5	6
		A Sugiyama	77	6											18	L Raymond	3	7	4
	Q	L Krasnoroutskaya	63	0					A Sugiyama	1	1r
								18	L Raymond	6	4
																						13	S Testud	3	1
																						2	L Davenport	6	6
								28	N Dechy	2	65
		É Loit	63	2					L Osterloh	6	77
		L Osterloh	77	6												L Osterloh	2	0
		R de los Ríos	4	4											2	L Davenport	6	6
		D Bedáňová	6	6					D Bedáňová	1	1r
								2	L Davenport	6	4

	First round							Qualifying competition
	1	Rachel McQuillan	3	0
	WC	Anikó Kapros	6	6				WC	Anikó Kapros	6	6
		Milagros Sequera	6	63	6				Milagros Sequera	2	1
	16	Amanda Hopmans	1	77	1

	First round							Qualifying competition
	2	Lina Krasnoroutskaya	6	6
		Wynne Prakusya	4	1				2	Lina Krasnoroutskaya	6	6
	WC	Sunitha Rao	3	6	6			WC	Sunitha Rao	1	2
	13	Allison Bradshaw	6	0	3

	First round							Qualifying competition
	3	Mariana Díaz Oliva	7	6
		Katalin Marosi-Aracama	5	1				3	Mariana Díaz Oliva	6	6
		Gréta Arn	6	6					Gréta Arn	2	3
	22	Olga Barabanschikova	3	3

	First round							Qualifying competition
	4	Evie Dominikovic	6	3	6
		Laurence Courtois	3	6	3			4	Evie Dominikovic	6	2	3
	WC	Jelena Janković	1	0r				23	María José Martínez Sánchez	1	6	6
	23	María José Martínez Sánchez	6	4

	First round							Qualifying competition
	5	Dawn Buth	6	1	3
		Erika deLone	3	6	6			Erika deLone		6	2	3
		Stéphanie Foretz	6	6				Stéphanie Foretz		4	6	6
	24	Sandra Načuk	4	0

	First round							Qualifying competition
	6	Lubomira Bacheva	6	2	6
		Janet Lee	3	6	2			6	Lubomira Bacheva	5	5
	WC	Tatiana Perebiynis	6	6				WC	Tatiana Perebiynis	7	7
	15	Selima Sfar	1	2

	First round							Qualifying competition
	7	Sandra Kleinová	5	1
		Katarina Srebotnik	7	6				Katarina Srebotnik		62	6	2
		Alicia Molik	6	6				Alicia Molik		77	1	6
	17	Ľudmila Cervanová	0	1

	First round							Qualifying competition
	8	Alina Jidkova	3	1
		Tara Snyder	6	1r				8	Alina Jidkova	2	7	64
	Alt	Maja Palaveršić	4	6	7			Alt	Maja Palaveršić	6	5	77
	20	Martina Suchá	6	2	5

	First round							Qualifying competition
	9	Kristie Boogert	6	4	3
		Els Callens	2	6	6				Els Callens	5	4
		Yuka Yoshida	4	2				19	Virginie Razzano	7	6
	19	Virginie Razzano	6	6

	First round							Qualifying competition
	10	Janette Husárová	6	4	4
		Yi Jing-Qian	4	6	6				Yi Jing-Qian	6	77
		Saori Obata	6	65	3r			21	Bianka Lamade	2	63
	21	Bianka Lamade	3	77	2

	First round							Qualifying competition
	11	María Vento	6	7
	WC	Vera Zvonareva	2	5				11	María Vento	1	4
		Anca Barna	3	2				18	Jill Craybas	6	6
	18	Jill Craybas	6	6

	First round							Qualifying competition
	12	Catalina Castaño	4	63
	WC	Bethanie Mattek	6	77				WC	Bethanie Mattek	5	4
		Lourdes Domínguez Lino	5	0				14	Sandra Cacic	7	6
	14	Sandra Cacic	7	6

• Official results archive (ITF)
• Official results archive (WTA)