The following highways are numbered 216:

• Alberta Highway 216
• Manitoba Provincial Road 216
• Prince Edward Island Route 216
• Quebec Route 216
• Nova Scotia Route 216

• China National Highway 216

• National Route 216

• National Highway 216 (India)

• A216 road
• B216 road

• U.S. Route 216 (former)
• Alabama State Route 216
• California State Route 216
• Connecticut Route 216
• Florida State Road 216 (former)
• Georgia State Route 216
• K-216 (Kansas highway)
• Kentucky Route 216
• Maine State Route 216
• Maryland Route 216
• M-216 (Michigan highway)
• Minnesota State Highway 216 (former)
• Montana Secondary Highway 216 (former)
• New Mexico State Road 216
• New York State Route 216
• North Carolina Highway 216
• Ohio State Route 216
• Oregon Route 216
• Pennsylvania Route 216
• Rhode Island Route 216
• South Carolina Highway 216
• Tennessee State Route 216
• Texas State Highway 216 (former)
  • Texas State Highway Loop 216 (former)
  • Texas State Highway Spur 216
• Utah State Route 216 (former)
• Virginia State Route 216
• Wyoming Highway 216