Kamilla Rakhimova won the singles title at the 2024 Guadalajara 125 Open, defeating Tatjana Maria in the final, 6–3, 6–7^(5–7), 6–3.^[1][2]

Sloane Stephens was the reigning champion from 2022, when the event was last held,^[3] but did not participate this year.

	Semifinals							Final
	1	Anna Karolína Schmiedlová	0	2
	4	Tatjana Maria	6	6
								4	Tatjana Maria	3	77	3
								5	Kamilla Rakhimova	6	65	6
		Emiliana Arango	4	2
	5	Kamilla Rakhimova	6	6

	First round							Second round							Quarterfinals							Semifinals
	1	AK Schmiedlová	4	6	6
	WC	J García Ruiz	6	2	0			1	AK Schmiedlová	6	6
	Q	K Birrell	6	7				Q	K Birrell	2	3
	WC	A Konjuh	2	5											1	AK Schmiedlová	6	6
	WC	D Pavlou	6	6											6	E Bektas	3	2
	Q	V Strakhova	1	2				WC	D Pavlou	1	1
		UM Arconada	710	66	1			6	E Bektas	6	6
	6	E Bektas	68	78	6																	1	AK Schmiedlová	0	2
	4	T Maria	79	6																		4	T Maria	6	6
		K Day	67	4				4	T Maria	7	6
		V Hu	6	62	4				A Eala	5	2
		A Eala	3	77	6										4	T Maria	7	6
		L Stefanini	6	6												O Gadecki	5	0
	Q	S Vickery	1	1					L Stefanini	1	6	4
		O Gadecki	6	6					O Gadecki	6	4	6
	8	J Riera	4	2

	First round							Second round							Quarterfinals							Semifinals
	9	M Joint	4	3
		E Mandlik	6	6					E Mandlik	4	6	2
	WC	AK Guadiana Campos	0	1					E Arango	6	3	6
		E Arango	6	6												E Arango	6	5	7
		AS Sánchez	3	3												R Marino	3	7	5
		A Sharma	6	6					A Sharma	3	69
		R Marino	6	6					R Marino	6	711
	3	R Zarazúa	4	2																			E Arango	4	2
	5	K Rakhimova	6	6																		5	K Rakhimova	6	6
	Q	S Murray Sharan	3	0				5	K Rakhimova	6	6
		E Chong	3	2					T Preston	1	2
		T Preston	6	6											5	K Rakhimova	6	3
		A Krunić	6	77											2	M Trevisan	1	1r
		M Stakusic	0	65					A Krunić	3	3
		V Savinykh	6	4	2			2	M Trevisan	6	6
	2	M Trevisan	3	6	6

	Qualifying competition
	1	Sachia Vickery	6	4
		Fanny Stollár	4	0r

	Qualifying competition
	2	Kimberly Birrell	6	6
	WC	María Fernanda Navarro Oliva	2	1

	Qualifying competition
	3	Valeriya Strakhova	6	6
	WC	Sandra Alonso Abril	1	0

	Qualifying competition
	Alt	Samantha Murray Sharan	3
		Kateryna Volodko	3r

• Main draw
• Qualifying draw