Camila Osorio defeated Marie Bouzková in the final, 6–3, 7–6^(7–5) to win the singles tennis title at the 2024 Copa Colsanitas. It was her second title at her home event, after 2021.

Tatjana Maria was the two-time defending champion,^[1] but lost to Osorio in the quarterfinals.

	Semifinals							Final
	1	Marie Bouzková	6	77
	8	Kamilla Rakhimova	4	62
								1	Marie Bouzková	3	65
								6	Camila Osorio	6	77
		Sara Errani	64	4
	6	Camila Osorio	77	6

	First round							Second round							Quarterfinals							Semifinals
	1	M Bouzková	6	6
		R Marino	0	3				1	M Bouzková	6	6
		T Zidanšek	2	6	3				H Baptiste	3	4
		H Baptiste	6	4	6										1	M Bouzková	2	6	7
	WC	Y Monroy	1	2											7	L Siegemund	6	2	5
		F Jones	6	6					F Jones	77	4	2
	Q	I Shymanovich	65	5				7	L Siegemund	64	6	6
	7	L Siegemund	77	7																		1	M Bouzková	6	77
	4	C Bucșa	6	7																		8	K Rakhimova	4	62
	Q	X You	1	5				4	C Bucșa	6	6
		J Niemeier	6	6					J Niemeier	4	1
	Q	N Brancaccio	2	3											4	C Bucșa	6	4	5
		R Zarazúa	6	6											8	K Rakhimova	0	6	7
	WC	M Higuita	0	0					R Zarazúa	64	77	3
	WC	V Mediorreal	4	78	1			8	K Rakhimova	77	65	6
	8	K Rakhimova	6	66	6

	First round							Second round							Quarterfinals							Semifinals
	5	N Podoroska	4	1
		L Pigossi	6	6					L Pigossi	6	63	4
	Q	K Zavatska	6	3	4				I Bara	3	77	6
		I Bara	2	6	6											I Bara	6	2	3
		Y Starodubtseva	2	3												S Errani	4	6	6
		S Errani	6	6					S Errani	6	4	6
		M Timofeeva	1	3				3	S Sorribes Tormo	2	6	4
	3	S Sorribes Tormo	6	6																			S Errani	64	4
	6	C Osorio	6	6																		6	C Osorio	77	6
	Q	M Stakusic	4	2				6	C Osorio	77	6
		L Stefanini	6	2	4			Q	A Todoni	64	4
	Q	A Todoni	4	6	6										6	C Osorio	1	6	6
		E Arango	0	3											2	T Maria	6	3	3
		J Riera	6	6					J Riera	6	0	2
		A Bondár	3	2				2	T Maria	2	6	6
	2	T Maria	6	6

	First round							Qualifying competition
	1	Suzan Lamens	6	6
	WC	María Torres Murcia	0	3
								1	Suzan Lamens	2	1
								12	Nuria Brancaccio	6	6
		Whitney Osuigwe	2	1
	12	Nuria Brancaccio	6	6

	First round							Qualifying competition
	2	Natalija Stevanović	6	6
		Victoria Hu	0	1
								2	Natalija Stevanović	1	4
								10	You Xiaodi	6	6
	WC	María Sánchez Uribe	1	1
	10	You Xiaodi	6	6

	First round							Qualifying competition
	3	Katarina Zavatska	6	7
		Victoria Rodríguez	1	5
								3	Katarina Zavatska	0	6	6
								7	Elvina Kalieva	6	3	3
		Jana Kolodynska	6	63	4
	7	Elvina Kalieva	1	77	6

	First round							Qualifying competition
	4	Iryna Shymanovich	3	6	6
	WC	María Paulina Pérez	6	1	0
								4	Iryna Shymanovich	6	6
								9	Harmony Tan	4	3
		Katarina Kozarov	0	0
	9	Harmony Tan	6	6

	First round							Qualifying competition
	5	Dalila Jakupović	7	3	3
		Fanny Stollár	5	6	6
									Fanny Stollár	60	0r
								8	Marina Stakusic	77	0
	WC	María Paz Ospina	2	2
	8	Marina Stakusic	6	6

	First round							Qualifying competition
	6	Carol Zhao	3	65
		Varvara Lepchenko	6	77
									Varvara Lepchenko	64	4
								11	Anca Todoni	77	6
		Ana Sofía Sánchez	4	4
	11	Anca Todoni	6	6

• Main draw
• Qualifying draw