Yui Kamiji defeated Aniek van Koot in the final, 6–2, 6–2 to win the women's singles wheelchair tennis title at the 2020 Australian Open.

Diede de Groot was the two-time defending champion, but was defeated by Zhu Zhenzhen in the quarterfinals.

	Quarterfinals							Semifinals							Final
	1	Diede de Groot	77	3	5
		Zhu Zhenzhen	63	6	7
									Zhu Zhenzhen	6	0	4
									Aniek van Koot	1	6	6
		Marjolein Buis	67	4
		Aniek van Koot	79	6
																Aniek van Koot	2	2
															2	Yui Kamiji	6	6
		Kgothatso Montjane	6	6
		Sabine Ellerbrock	0	1
									Kgothatso Montjane	3	5
								2	Yui Kamiji	6	7
		Jordanne Whiley	2	65
	2	Yui Kamiji	6	77

• Drawsheet on ausopen.com