Four-time defending champion Rafael Nadal successfully defended his title, defeating David Ferrer in a rematch of the previous year's final, 6–2, 7–5, to win the singles title at the 2009 Barcelona Open. It was his record-extending fifth title at the Barcelona Open.

The top eight seeds receive a bye into the second round.

	Quarterfinals							Semifinals							Final
	1	Rafael Nadal	w/o
	7	David Nalbandian
								1	Rafael Nadal	6	6
								3	Nikolay Davydenko	3	2
	3	Nikolay Davydenko	64	6	6
	9	Radek Štěpánek	7	2	2
															1	Rafael Nadal	6	7
															4	David Ferrer	2	5
	6	Tommy Robredo	3	4
	4	David Ferrer	6	6
								4	David Ferrer	2	6	7
								5/WC	Fernando González	6	2	65
	5/WC	Fernando González	6	4	6
	2	Fernando Verdasco	3	6	4

	First round							Second round							Third round							Quarterfinals
								1	R Nadal	6	6
	Q	F Gil	6	6				Q	F Gil	2	2
		Y-h Lu	2	1											1	R Nadal	6	6
		C Rochus	6	6												C Rochus	2	0
		N Devilder	4	4					C Rochus	6	62	6
		J Hernych	6	3	2			13	R Gasquet	1	7	3
	13	R Gasquet	4	6	6																	1	R Nadal	w/o
	10	N Almagro	3	7	2																	7	D Nalbandian
		V Hănescu	6	67	2r			10	N Almagro	3	7	6
		JI Chela	6	3	6				JI Chela	6	64	3
		T Bellucci	2	6	4										10	N Almagro	3	4
	WC	JC Ferrero	5	63											7	D Nalbandian	6	6
		I Kunitsyn	7	7					I Kunitsyn	5	4
								7	D Nalbandian	7	6

	First round							Second round							Third round							Quarterfinals
								3	N Davydenko	7	6
		G Cañas	60	3					A Clément	62	2
		A Clément	7	6											3	N Davydenko	7	3	6
		M Granollers	63	3											16	F López	68	6	4
	Q	S Ventura	7	6				Q	S Ventura	6	4	3
		J Nieminen	2	1				16	F López	2	6	6
	16	F López	6	6																		3	N Davydenko	64	6	6
	9	R Štěpánek	6	2	6																	9	R Štěpánek	7	2	2
		G García López	4	6	4			9	R Štěpánek	7	6
		A Montañés	4	61					J Tipsarević	62	0
		J Tipsarević	6	7											9	R Štěpánek	6	1	7
		M Youzhny	65	7	6										8	S Wawrinka	4	6	5
		V Troicki	7	66	3				M Youzhny	5	1
								8	S Wawrinka	7	6

	First round							Second round							Third round							Quarterfinals
								6	T Robredo	7	6
		D Junqueira	4	6	4			WC	G Gaudio	66	1
	WC	G Gaudio	6	3	6										6	T Robredo	4	6	6
		M Gicquel	3	4											12	I Andreev	6	2	3
	WC	A Martín	6	6				WC	A Martín	2	2
		A Seppi	3	6	5			12	I Andreev	6	6
	12	I Andreev	6	3	7																	6	T Robredo	3	4
	14	R Söderling	6	6																		4	D Ferrer	6	6
		T Gabashvili	1	4				14	R Söderling	65	6	2
		E Gulbis	6	3	3				P Starace	7	2	6
		P Starace	4	6	6											P Starace	6	5	2
	Q	P Riba	2	65											4	D Ferrer	4	7	6
	Q	M Kukushkin	6	7				Q	M Kukushkin	3	6	1
								4	D Ferrer	6	3	6

	First round							Second round							Third round							Quarterfinals
								5/WC	F González	6	6
		D Sela	4	5					J Acasuso	4	4
		J Acasuso	6	7											5/WC	F González	7	6
	Q	F Fognini	1	3												J Mónaco	66	3
	Q	D Gimeno Traver	6	6				Q	D Gimeno Traver	5	4
		J Mónaco	6	6					J Mónaco	7	6
	11/WC	M Safin	4	1																		5/WC	F González	6	4	6
	15	T Berdych	5																			2	F Verdasco	3	6	4
		M Vassallo Argüello	2	r				15	T Berdych	6	4	6
		Ó Hernández	6	6					Ó Hernández	2	6	3
		E Schwank	2	4											15	T Berdych	5	7	4
	Q	N Lapentti	6	2	6										2	F Verdasco	7	5	6
		P-H Mathieu	4	6	2			Q	N Lapentti	5	3
								2	F Verdasco	7	6

	First round							Qualifying competition
	1	Fred Gil	6	6
		Laurent Recouderc	1	1
								1	Fred Gil	6	2	6
									Daniel Muñoz de la Nava	3	6	1
		Daniel Muñoz de la Nava	4	6	6
	12	Rubén Ramírez Hidalgo	6	3	0

	First round							Qualifying competition
	2	Mischa Zverev	6	3	6
		Łukasz Kubot	4	6	1
								2	Mischa Zverev	4	77	3
								14	Santiago Ventura Bertomeu	6	63	6
		Miguel Ángel López Jaén	4	2
	14	Santiago Ventura Bertomeu	6	6

	First round							Qualifying competition
	3	Daniel Gimeno Traver	3	77	6
		Stéphane Bohli	6	64	2
								3	Daniel Gimeno Traver	3	77	7
								WC	Albert Ramos Viñolas	6	63	5
	WC	Albert Ramos Viñolas	6	6
	8	Iván Navarro	3	4

	First round							Qualifying competition
	4	Nicolás Massú	4	4
		Pere Riba	6	6
									Pere Riba	6	77
								13	Flavio Cipolla	2	65
		Josselin Ouanna	1	4
	13	Flavio Cipolla	6	6

	First round							Qualifying competition
	5	Paul Capdeville	1	2
	WC	Marc López	6	6
								WC	Marc López	7	2	1
								9	Nicolás Lapentti	5	6	6
		Pablo Cuevas	6	3	0
	9	Nicolás Lapentti	2	6	6

	First round							Qualifying competition
	6	Pablo Andújar	6	6
	WC	David Marrero	3	2
								6	Pablo Andújar	4	6	2
									Mikhail Kukushkin	6	4	6
		Mikhail Kukushkin	77	6
	10	Andrey Golubev	65	4

	First round							Qualifying competition
	7	Kristof Vliegen	1	6	6
	WC	Guillermo Alcaide	6	3	3
								7	Kristof Vliegen	6	3	4
								11	Fabio Fognini	2	6	6
		Lamine Ouahab	6	2	68
	11	Fabio Fognini	1	6	710

• Main Draw
• Qualifying Draw