Room 101 (pronounced one-o-one) refers to a torture chamber in the Ministry of Love in George Orwell's novel Nineteen Eighty-Four.

Room 101 may also refer to:

• Room 101 (radio series), BBC Radio 5 (1992–1994) and BBC Radio 4 (2023–) programme
• Room 101 (British TV series), BBC Two (1994–2007) and BBC One (2012–2018) programme
• Room 101 (Australian TV series) (2015), SBS One comedy show

• Room 101 (1982 mixtape) by The Faction
• Room 101 (1984 song) by the Eurythmics from the album 1984 (For the Love of Big Brother)
• Room 101 (2000 song) from the album Drama (Jamelia album)
• Room 101 (2002 song) from the album Funk (album)
• Room 101 (2017 song) by Tich
• Room 101 (2021 album) by Karmamoi

• Room 101 (brand), a brand of cigars produced by Forged Cigars
• Room 101 (alias), an alias used by vexatious gamer group Patriotic Nigras in Second Life

• All pages with titles beginning with room 101
• All pages with titles containing room 101
• 101 (disambiguation)