The following highways are numbered 401:

• British Columbia Highway 401 (former)
• Manitoba Provincial Road 401
• Ontario Highway 401

• National Route 401

• Road to El Fe (4–401)

• Japan National Route 401

• Norwegian County Road 401

• Thailand Route 401

• U.S. Route 401
• Florida:
  • Florida State Road 401
  • County Road 401 (Brevard County, Florida)
• Georgia State Route 401 (unsigned designation for Interstate 75)
• Kentucky Route 401
• Louisiana Highway 401
• Nevada State Route 401
• New York:
  • New York State Route 401 (former)
  • County Route 401 (Albany County, New York)
• North Carolina Highway 401 (former)
• Pennsylvania Route 401
  • Pennsylvania Route 401 Alternate Truck
• Puerto Rico Highway 401
• Rhode Island Route 401
• Virginia State Route 401
• Washington State Route 401