The following highways are numbered 328:

- Cameron Drive (No through road)

• Nova Scotia Route 328

• China National Highway 328

• National Route 328

• Japan National Route 328

• Arkansas Highway 328
• Florida:
  • Florida State Road 328 (former)
  • County Road 328 (Marion County, Florida)
• Georgia State Route 328
• Kentucky Route 328
• Louisiana Highway 328
• Maryland Route 328
• Mississippi Highway 328
• Montana Secondary Highway 328
• New York:
  • New York State Route 328
    • New York State Route 328A (former)
  • County Route 328 (Erie County, New York)
• Ohio State Route 328
• Pennsylvania Route 328
• Puerto Rico Highway 328
• Tennessee State Route 328
• Texas:
  • Texas State Highway 328 (former)
  • Texas State Highway Loop 328
  • Farm to Market Road 328
• Virginia State Route 328
  • Virginia State Route 328 (former)