Route 241 or Highway 241 may refer to:

• Manitoba Provincial Road 241
• Prince Edward Island Route 241
• Quebec Route 241

• National Route 241

• Pinar del Rio–Vinales Road (1–241)
• Cotorro–Jaruco Road (2–241)

• Japan National Route 241

• U.S. Route 241 (former)
  • U.S. Route 241 (1926)
  • U.S. Route 241 (1930s)
• Alabama State Route 241
• Arkansas Highway 241
• California State Route 241
• Florida State Road 241 (former)
• Georgia State Route 241
• Indiana State Road 241
• Kentucky Route 241
• Maryland Route 241 (former)
• Minnesota State Highway 241
• Montana Secondary Highway 241
• New Mexico State Road 241
• New York State Route 241
• North Carolina Highway 241
• Ohio State Route 241
• Oregon Route 241
• Pennsylvania Route 241
• Tennessee State Route 241
• Texas State Highway 241 (former)
  • Texas State Highway Spur 241
  • Farm to Market Road 241
• Utah State Route 241
• Virginia State Route 241
• Washington State Route 241
• Wisconsin Highway 241
• Wyoming Highway 241