Route 165, or Highway 165, may refer to:

• New Brunswick Route 165
• Prince Edward Island Route 165
• Quebec Route 165
• Saskatchewan Highway 165
• Winnipeg Route 165

• National Route 165

• National Highway 165 (India)

• Japan National Route 165

• A165 road
• B165 road

• Interstate 165 (disambiguation)
• U.S. Route 165
• Alabama State Route 165
• California State Route 165
• Colorado State Highway 165
• Connecticut Route 165
• Florida State Road 165 (former)
• Georgia State Route 165
• Illinois Route 165
• Indiana State Road 165
• Iowa Highway 165
• Kentucky Route 165
• Maryland Route 165
• M-165 (Michigan highway)
• Minnesota State Highway 165 (former)
• Missouri Route 165
• Nevada State Route 165
• New Jersey Route 165
• New Mexico State Road 165
• New York State Route 165
• Ohio State Route 165
• Oklahoma State Highway 165
• Rhode Island Route 165
• South Carolina Highway 165
• Tennessee State Route 165
• Texas State Highway 165
  • Texas State Highway Spur 165
  • Ranch to Market Road 165
• Utah State Route 165
• Virginia State Route 165
• Washington State Route 165
• Wisconsin Highway 165

Territories:

• Puerto Rico Highway 165
  • Puerto Rico Highway 165R