Dafne may refer to:
Arts
- Dafne, a 1597 opera by Jacopo Peri
- La Dafne, a 1608 opera by Marco da Gagliano
- Dafne (Opitz–Schütz), a 1627 opera by Martin Opitz and Heinrich Schütz
Other uses
- Dafne (given name)
- DAFNE, a particle collider in Italy
- Dose adjustment for normal eating, a strategy in insulin therapy
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