The 2016 Tallahassee Tennis Challenger was a professional tennis tournament played on green clay courts. It was the 17th edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Tallahassee, Florida, United States between April 25 and May 1, 2016.

Country	Player	Rank1	Seed
USA	Donald Young	84	1
AUT	Gerald Melzer	112	2
USA	Tim Smyczek	119	3
USA	Bjorn Fratangelo	126	4
GEO	Nikoloz Basilashvili	130	5
ARG	Facundo Argüello	143	6
USA	Jared Donaldson	147	7
USA	Dennis Novikov	148	8

• ¹ Rankings as of April 18, 2016

The following players received wildcards into the singles main draw:

• Benjamin Lock
• Brian Baker
• Eric Quigley
• Tennys Sandgren

The following players received entry into the singles main draw as special exempts:

• Ernesto Escobedo
• Nicolás Jarry

The following players received entry from the qualifying draw:

• Sekou Bangoura
• Emilio Gómez
• James McGee
• Stefan Kozlov

• Quentin Halys def. Frances Tiafoe, 6–7^(6–8), 6–4, 6–2

• Dennis Novikov / Julio Peralta def. Peter Luczak / Marc Polmans, 3–6, 6–4, [12–10]

• Combo Main Draw

• Official Website