Statistics of Empress's Cup in the 2016 season.

It was contested by 48 teams, and INAC Kobe Leonessa won the championship.^[1]

• Hiroshima Bunkyo Women's University High School 0-3 Clubfields Linda
• Fujieda Junshin High School 1-2 Sendai University
• Tokuyama University 0-5 Nippatsu Yokohama FC Seagulls
• JFA Academy Fukushima 8-1 Shikoku Gakuin University
• NGU Nagoya FC 0-2 Orca Kamogawa FC
• Norddea Hokkaido 1-4 Hinomoto Gakuen High School
• Shizuoka Sangyo University 1-0 Urawa Reds Youth
• AS Harima ALBION 4-1 Nippon TV Menina
• Kamimura Gakuen High School 3-2 Seiwa Gakuen High School
• Cerezo Osaka Sakai 3-1 Fukui University of Technology Fukui High School
• Fukuoka J. Anclas 0-5 Waseda University
• Bunnys Kyoto SC 1-0 University of Tsukuba
• Toyo University 3-0 Japan Soccer College
• Mashiki Renaissance Kumamoto FC 3-2 Tokoha University Tachibana High School
• Tokyo International University 1-0 Angeviolet Hiroshima
• Daisho Gakuen High School 1-0 Niigata University of Health and Welfare

• Nippon TV Beleza 12-0 Clubfields Linda
• Ehime FC 5-0 Sendai University
• Konomiya Speranza Osaka-Takatsuki 1-3 Nippatsu Yokohama FC Seagulls
• JEF United Chiba 4-0 JFA Academy Fukushima
• Albirex Niigata 4-1 Orca Kamogawa FC
• Chifure AS Elfen Saitama 3-1 Hinomoto Gakuen High School
• Nippon Sport Science University 5-0 Shizuoka Sangyo University
• AC Nagano Parceiro 5-1 AS Harima ALBION
• Vegalta Sendai 7-0 Kamimura Gakuen High School
• Sfida Setagaya FC 3-0 Cerezo Osaka Sakai
• Nojima Stella Kanagawa Sagamihara 3-2 Waseda University
• Okayama Yunogo Belle 1-0 Bunnys Kyoto SC
• Iga FC Kunoichi 4-0 Toyo University
• Urawa Reds 6-0 Mashiki Renaissance Kumamoto FC
• Kibi International University 2-2 (pen 5-4) Tokyo International University
• INAC Kobe Leonessa 1-0 Daisho Gakuen High School

• Nippon TV Beleza 8-1 Ehime FC
• Nippatsu Yokohama FC Seagulls 1-2 JEF United Chiba
• Albirex Niigata 1-0 Chifure AS Elfen Saitama
• Nippon Sport Science University 1-2 AC Nagano Parceiro
• Vegalta Sendai 5-1 Sfida Setagaya FC
• Nojima Stella Kanagawa Sagamihara 3-1 Okayama Yunogo Belle
• Iga FC Kunoichi 0-2 Urawa Reds
• Kibi International University 0-3 INAC Kobe Leonessa

• Nippon TV Beleza 1-0 JEF United Chiba
• Albirex Niigata 2-0 AC Nagano Parceiro
• Vegalta Sendai 2-0 Nojima Stella Kanagawa Sagamihara
• Urawa Reds 0-1 INAC Kobe Leonessa

• Nippon TV Beleza 0-1 Albirex Niigata
• Vegalta Sendai 1-3 INAC Kobe Leonessa

• INAC Kobe Leonessa 0-0 (pen 5-4) Albirex Niigata

INAC Kobe Leonessa won the championship.